- The second semi-final took place in Oslo on 27 May 2010.
- The ten countries in this semi-final with the highest scoring points, according to a combination of televotes and jury votes from each voting country, qualified for the final.
- Norway and the United Kingdom voted in this semi-final.
| Draw | Country | Language | Artist | Song | Place | Points |
|---|---|---|---|---|---|---|
| 01 | Lithuania | English | InCulto | “Eastern European Funk” | 12 | 44 |
| 02 | Armenia | English | Eva Rivas | “Apricot Stone” | 6 | 83 |
| 03 | Israel | Hebrew | Harel Skaat | “Milim” (מילים) | 8 | 71 |
| 04 | Denmark | English | Chanée and N’evergreen | “In a Moment Like This” | 5 | 101 |
| 05 | Switzerland | French | Michael von der Heide | “Il pleut de l’or” | 17 | 2 |
| 06 | Sweden | English | Anna Bergendahl | “This Is My Life” | 11 | 62 |
| 07 | Azerbaijan | English | Safura | “Drip Drop” | 2 | 113 |
| 08 | Ukraine | English | Alyosha | “Sweet People” | 7 | 77 |
| 09 | Netherlands | Dutch | Sieneke | “Ik ben verliefd (Sha-la-lie)” | 14 | 29 |
| 10 | Romania | English | Paula Seling and Ovi | “Playing with Fire” | 4 | 104 |
| 11 | Slovenia | Slovene | Ansambel Žlindra and Kalamari | “Narodnozabavni rock” | 16 | 6 |
| 12 | Ireland | English | Niamh Kavanagh | “It’s for You” | 9 | 67 |
| 13 | Bulgaria | Bulgarian, English | Miro | “Angel si ti” (Ангел си ти) | 15 | 19 |
| 14 | Cyprus | English | Jon Lilygreen and The Islanders | “Life Looks Better in Spring” | 10 | 67 |
| 15 | Croatia | Croatian | Feminnem | “Lako je sve” | 13 | 33 |
| 16 | Georgia | English | Sofia Nizharadze | “Shine” | 3 | 106 |
| 17 | Turkey | English | maNga | “We Could Be the Same” | 1 | 118 |